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STEMCELL Technologies Inc pneumacult tm -ex plus medium
Pneumacult Tm Ex Plus Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pneumacult+tm+-+ex+plus+medium/pneumacult+ex+plus+medium/pmc12197413-66-15-20
Average 90 stars, based on 1 article reviews
pneumacult tm -ex plus medium - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Therapeutic strategies to reverse cigarette smoke-induced ion channel and mucociliary dysfunction in COPD airway epithelial cells
Article Snippet: Primary P0 normal HBEC and COPD-HBEC were expanded in PneumaCult TM -Ex Plus Medium (Cat. No. 05040; STEMCELL Technologies, Cambridge, MA, USA) supplemented with 50 μg/mL gentamycin (Cat. No. 15710–064; ThermoFisher Scientific) and 2.5 μg/mL amphotericin B (Cat. No. 15290–026; ThermoFisher Scientific).

Article Title: A Versatile Reporter Platform for Evaluating HDR- and NHEJ-Based Genome Editing in Airway Epithelial Cell Cultures Using an rAAV Vector
Article Snippet: Proliferating cultures: CuFi-8 cells and primary ferret airway epithelial cells were cultured and passaged in PneumaCult TM -Ex Plus medium (StemCell Technologies, Vancouver, BC, Canada) on plastic plates or dishes pre-coated with collagen IV (Sigma, St. Louis, MO, USA).

Article Title: Melatonin Vapes Contain Potential Contaminants and Alter the Human Bronchial Epithelial Transcriptome
Article Snippet: Cell Culture Primary human bronchial epithelial cells (hBECs) from non-diseased, non-smoker adult donors were obtained from the Marsico Lung Institute Tissue Procurement and Cell Culture Core (MLI TPC) at the University of North Carolina at Chapel Hill.1, 2 Cells were plated on bovine type-1 collagen-coated T75 flasks at a density of 1.75 million in PneumaCult TM-Ex Plus Medium (STEMCELL Technologies, Vancouver, Canda) supplemented with penicillin (100 U/ml) and streptomycin (100 ug/ml).

Article Title: Protocol For Differentiating Primary Human Small Airway Epithelial Cells at the Air-liquid Interface
Article Snippet: SAECs are seeded onto culture inserts in PneumaCult TM - Ex Plus medium (supplemented as above), airlifted after reaching 100% confluency and switched to PneumaCult TM -ALI-S medium (STEMCELL Technologies, Cat# 05050) supplemented with 0.48 μg/mL of hydrocortisone (STEMCELL Technologies, Cat# 07925), 2 ng/mL of heparin (STEMCELL Technologies, Cat# 07980) and 100 units/mL penicillin, 100 μg/mL streptomycin and 0.25 μg/mL amphotericin B (Gibco, Cat# 15240062).

Article Title: The response of nasal epithelial cells exposed to novel Lactobacillus and alpha-haemolytic Streptococcus isolated from the upper respiratory tract of children.
Article Snippet: Aims: To in v estigate the response of primary nasal epithelial cells (NECs) to no v el alpha haemolytic Streptococcus and lactobacilli strains, isolated from the upper respiratory tract of children.. Methods and results: Submerged cultures of NECs from healthy adult donors w ere e xposed to either no v el strains; Lactobacillus rhamnosus D3189, D3160, Streptococcus salivarius D3837; or commercially available probiotic strains L. rhamnosus LB21, S. salivarius K12; or a pathogenic strain ( S. pneumoniae 49619).. Cytotoxicity (measured through lactate deh y drogenase release) and cytokine release were quantified 24 hours post-e xposure.

Article Title: A Versatile Reporter Platform for Evaluating HDR- and NHEJ-Based Genome Editing in Airway Epithelial Cell Cultures Using an rAAV Vector
Article Snippet: Seeding occurred in PneumaCult TM -Ex Plus medium (StemCell Technologies).

Cell Culture:

Article Title: Single-stranded DNA with internal base modifications mediates highly efficient knock-in in primary cells using CRISPR-Cas9
Article Snippet: .. Human ABCs were cultured in PneumaCult TM Ex Plus medium (StemCell Technologies #05040) with addition of ROCK inhibitor 10 μM (Y-27632, MedChemExpress # HY-10583) and iMatrix-511 (Nacalai USA #892021) at 5000–10 000 cells/cm 2 in tissue culture flasks. .. G-CSF Mobilized Human Peripheral Blood CD34 + Cells were purchased from StemCell Technologies (Catalog: 70060.1) and the Hematopoietic Cell Procurement and Resource Development Center at The Fred Hutchison Cancer Center.



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